Magnetic Micropallets for Single Adherent Cell Recovery and Analysis
نویسندگان
چکیده
This paper presents a novel microtechnology, magnetic micropallets, for the recovery of single adherent cells from large mixed populations. This work significantly advances the base technology, the micropallet array, which itself is effective for the analysis of adherent cell populations and isolation and selection of individual cells. We greatly improved the method of recovery of individual micropallets by incorporating ferromagnetic iron oxide nanoparticles into their structure such that single micropallets can be manipulated and collected magnetically.
منابع مشابه
Ferromagnetic micropallets for magnetic capture of single adherent cells.
We present a magnetic micropallet array and demonstrate its capacity to recover specific, individual adherent cells from large populations and deliver them for downstream single cell analysis. A ferromagnetic photopolymer was formulated, characterized, and used to fabricate magnetic micropallets, which are microscale pedestals that provide demarcated cell growth surfaces with preservation of bi...
متن کاملGlass Micropallets for Adherent Cell Analysis and Recovery
This paper presents a novel microtechnology, glass micropallets, for the recovery of single adherent cells from mixed cellular populations. This work significantly advances the base technology, the micropallet array, which has been demonstrated for the analysis of adherent cell populations and isolation and selection of individual cells. We report a complete departure from the photolithographic...
متن کاملPhenotypic and Functional Comparison between Flask Adherent and Magnetic Activated Cell Sorted Monocytes Derived Dendritic Cells
Background: Generation of an effective dendritic cell (DC) based cancer vaccine depends on appropriate differentiation of monocytes in vitro. Objective: To compare the effects of monocyte separation methods, flask adherence (Flask-DC) and magnetic activated cell sorting (MACS-DC), on phenotypic and functional characteristics of resultant DCs. Methods: DCs from healthy volunteers were generated ...
متن کاملMulticolor Immunofluorescent Imaging of Complex Cellular Mixtures on Micropallet Arrays Enables the Identification of Single Cells of Defined Phenotype.
A Micropallet-Array-based strategy allowing the identification of cells of defined phenotype in complex mixtures, such as would be obtained from a tissue biopsy, is presented. Following the distribution of single adherent cells from the mixture on individual pedestals, termed "micropallets", immunofluorescent confocal imaging is applied to interrogate the expression of five cell surface molecules.
متن کاملFunctionalization of Micropallet Arrays for Multichannel Immunofluorescent Imaging of Complex Cellular Mixtures
The micropallet array is a photoresist-based platform that provides a means to isolate and recover single adherent cells on individual pedestals, termed “micropallets”. We present a strategy to functionalize the micropallet array by incorporating multichannel immunofluorescent confocal imaging to identify cellular subsets of defined surface phenotype within complex heterogeneous mixtures, such ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2010